{"product_id":"wide-range-elisa-kit-for-glycated-hemoglobin-a1c-hba1c-wea190mu","title":"Wide-range ELISA Kit for Glycated Hemoglobin A1c (HbA1c) - WEA190Mu","description":"\u003cp\u003eWide-range ELISA Kit for Glycated Hemoglobin A1c (HbA1c)\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: WEA190Mu-24, WEA190Mu-48, WEA190Mu-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series: Wide range kit\u003c\/p\u003e\n\n\u003cp\u003eTarget: Glycated Hemoglobin A1c\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Mouse\u003c\/p\u003e\n\n\u003cp\u003eFeatured Series Function: Detects protein (wildest detection range for analytes from different samples type)\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Reactive with Mouse HbA1c \/ Glycated Hemoglobin A1c\u003c\/p\u003e\n\n\u003cp\u003eMethod: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eDetection principle: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eDetection\u003cbr\u003e\nrange: 12.35-1000ug\/mL\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 5.23ug\/mL\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 3h\u003c\/p\u003e\n\n\u003cp\u003eSample Size: 50uL\u003c\/p\u003e\n\n\u003cp\u003eRecommended\/Predicted\u003cbr\u003e\nSample Types: Plasma and Erythrocyte Lysates\u003c\/p\u003e\n\n\u003cp\u003eAssay Precision: Intra-Assay: CV\u0026lt;10%, Inter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\n\u003cp\u003eStorage: 4°C for 1 month\/ -20°C for long-term(One year within shelf life)\u003c\/p\u003e\n\n\u003cp\u003eShelf-life: 12 months\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Glycated Hemoglobin A1c (HbA1c).\u003cbr\u003e\nNo significant cross-reactivity or interference between Wide-range Glycated Hemoglobin A1c (HbA1c) and analogues was observed.\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Glycated Hemoglobin A1c (HbA1c) were tested 20 times on one plate, respectively.\u003cbr\u003e\nInter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Glycated Hemoglobin A1c (HbA1c) were tested on 3 different plates, 8 replicates in each plate.\u003cbr\u003e\nCV(%) = SD\/meanX100\u003c\/p\u003e\n\n\u003cp\u003eStability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003e\nTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\u003c\/p\u003e\n\n\u003cp\u003eTarget: 1. Prepare all reagents, samples and standards;\u003cbr\u003e\n2. Add 50µL standard or sample to each well.\u003cbr\u003e\n And then add 50µL prepared Detection Reagent A immediately.\u003cbr\u003e\n Shake and mix. Incubate 1 hour at 37°C;\u003cbr\u003e\n3. Aspirate and wash 3 times;\u003cbr\u003e\n4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;\u003cbr\u003e\n5. Aspirate and wash 5 times;\u003cbr\u003e\n6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;\u003cbr\u003e\n7. Add 50µL Stop Solution. Read at 450 nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eTest principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Glycated Hemoglobin A1c (HbA1c) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Glycated Hemoglobin A1c (HbA1c) and unlabeled Wide-range Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Wide-range Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Glycated Hemoglobin A1c (HbA1c) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Glycated Hemoglobin A1c (HbA1c) in the sample.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"Cloud-Clone","offers":[{"title":"24T","offer_id":45383825621171,"sku":"WEA190Mu-24","price":352.8,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":45383825653939,"sku":"WEA190Mu-48","price":617.4,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45383825686707,"sku":"WEA190Mu-96","price":882.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/Cloud-Clone.jpg?v=1774117629","url":"https:\/\/afsbio.com\/products\/wide-range-elisa-kit-for-glycated-hemoglobin-a1c-hba1c-wea190mu","provider":"AFSBio Inc.","version":"1.0","type":"link"}