{"product_id":"wnt-1-g331-polyclonal-antibody-bs1777","title":"Wnt-1 (G331) Polyclonal Antibody-BS1777","description":"\u003cp\u003eWnt-1 (G331) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS1777-50, BS1777-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P04628\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC, IF\u003c\/p\u003e \n\n\u003cp\u003eBackground: Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue.\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Wnt-1 (G331) polyclonal antibody detects endogenous levels of Wnt-1 protein.\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of Wnt-1 (G331) polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane 1: HEK293T whole cell lysate (40ug)\u003cbr\u003e\nLane 2: Hela whole cell lysate (40ug)\u003cbr\u003e\nLane 3: DLD whole cell lysate (40ug)\u003cbr\u003e\nLane 4: The Testis tissue lysate of Mouse (40ug)\u003cbr\u003e\nLane 5: The Testis tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Wnt-1 (G331) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e \n\n\u003cp\u003eBioworld Molecular Weight: ~ 41 kDa\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1; INT1; WNT 1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42953215180979,"sku":"BS1777-50","price":254.38,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42953215213747,"sku":"BS1777-100","price":442.75,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_adb2be29-c336-43db-ac0d-bfb926896ef2.jpg?v=1705056915","url":"https:\/\/afsbio.com\/products\/wnt-1-g331-polyclonal-antibody-bs1777","provider":"AFSBio Inc.","version":"1.0","type":"link"}