{"product_id":"wt1-as-polyclonal-antibody-bs60050","title":"WT1-AS Polyclonal Antibody-BS60050","description":"\u003cp\u003eWT1-AS Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS60050-50, BS60050-100\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: Putative Wilms tumor upstream neighbor 1 gene protein; WIT-1; Wilms tumor-associated antisense RNA; WT1-AS; WIT1\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: Q06250\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB\u003c\/p\u003e \n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003c\/p\u003e\n\n\u003cp\u003eBackground: Wilms’ tumor is a neoplasm of the kidneys that usually occurs in children and is characterized by the presence of abortive tubules and glomeruli surrounded by a spindled cell stroma. The 11p13 Wilms’ tumor locus consists of two coordinately regulated transcripts, WT1 and WIT-1, which are mutated in Wilms’ tumors. Wilms’ tumor upstream neighbor 1 (WIT-1) is encoded by an intron-less gene upstream of the Wilms’ tumor 1 (WT1) gene, which is is important for nephrogenesis and gonadal growth. The WT1 gene is bi-directionally transcribed from the same promoter region as WIT-1, which may function as an antisense regulator of WT1. WIT-1 and WT1 have the same temporal and cell-restricted expression pattern, although the expression of WIT-1 is less abundant. Methylation of the WIT-1 gene is implicated in hematologic malignancy of chemoresistant acute myeloid leukemia. Single nucleotide polymorphisms (SNPs) in the WIT-1 gene are significantly associated with focal segmental glomerulosclerosis.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: WT1-AS polyclonal antibody detects endogenous levels of WT1-AS protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 15 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of WT1-AS polyclonal antibody at 1:500 dilution\u003cbr\u003e\nLane 1: A549 whole cell lysate\u003cbr\u003e\nLane 2: Raw264.7 whole cell lysate\u003cbr\u003e\nLane 3: H9C2 whole cell lysate\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: A synthetic peptide corresponding to residues in Human WT1-AS.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42911826935987,"sku":"BS60050-50","price":260.23,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42911826968755,"sku":"BS60050-100","price":452.94,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_34811ce3-1d5c-40af-aa5d-97dc06699790.jpg?v=1703180200","url":"https:\/\/afsbio.com\/products\/wt1-as-polyclonal-antibody-bs60050","provider":"AFSBio Inc.","version":"1.0","type":"link"}