{"product_id":"xrcc4-rabbit-polyclonal-antibody-es3716","title":"XRCC4 Rabbit Polyclonal Antibody - ES3716","description":"\u003cp\u003eXRCC4 Rabbit Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50μL, 100μL\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ES3716-50, ES3716-100\u003c\/p\u003e\n\n\u003cp\u003eLead times: 1-2 weeks, if manufacturer has product in stock\u003c\/p\u003e\n\n\u003cp\u003eManufacturer\/Ship Location: China\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015],\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Rat, Mouse,\u003c\/p\u003e \n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ELISA\u003c\/p\u003e\n\n\u003cp\u003eAlternate Names: XRCC4, DNA repair protein XRCC4, X-ray repair cross-complementing protein 4\u003c\/p\u003e\n\n\u003cp\u003eClonality: Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: The antiserum was produced against synthesized peptide derived from human XRCC4. AA range:261-310\u003c\/p\u003e\n\n\u003cp\u003eIsotype: IgG\u003c\/p\u003e\n\n\u003cp\u003eTarget: XRCC4\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: XRCC4 Polyclonal Antibody detects endogenous levels of XRCC4 protein.\u003c\/p\u003e\n\n\u003cp\u003eDilution: Western Blot: 1\/500 - 1\/2000. ELISA: 1\/20000. Not yet tested in other applications.\u003c\/p\u003e\n\n\u003cp\u003ePurification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.\u003c\/p\u003e\n\n\u003cp\u003eConcentration: 1 mg\/ml,\u003c\/p\u003e\n\n\u003cp\u003eFormlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.\u003c\/p\u003e\n\n\u003cp\u003eExpression: Widely expressed,\u003c\/p\u003e\n\n\u003cp\u003eObserved Band (KD): 40kD\u003c\/p\u003e\n\n\u003cp\u003eFields: Non-homologous end-joining\u003c\/p\u003e\n\n\u003cp\u003eFunction: Function: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends\u003cbr\u003e\nPTM: Monoubiquitinated\u003cbr\u003e\nPTM: Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX\u003cbr\u003e\nPTM: Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination\u003cbr\u003e\nSimilarity: Belongs to the XRCC4 family\u003cbr\u003e\nSubunit: Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dep\u003c\/p\u003e\n\n\u003cp\u003eSubcellular Location: Nucleus Chromosome Localizes to site of double-strand breaks, [Protein XRCC4, C-terminus]: Cytoplasm Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3)\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: -20°C\/1 year\u003c\/p\u003e\n\n\u003cp\u003eGene Name: XRCC4\u003c\/p\u003e\n\n\u003cp\u003eProtein Name: DNA repair protein XRCC4\u003c\/p\u003e\n\n\u003cp\u003eMouse SwissProt Number: Q924T3\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"50μL","offer_id":46316502483123,"sku":"ES3716-50","price":186.0,"currency_code":"CAD","in_stock":true},{"title":"100μL","offer_id":46316502515891,"sku":"ES3716-100","price":372.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/elk_antibodies_d75295e2-ffc1-4e0f-a3e7-87715c8983a9.jpg?v=1793190507","url":"https:\/\/afsbio.com\/products\/xrcc4-rabbit-polyclonal-antibody-es3716","provider":"AFSBio Inc.","version":"1.0","type":"link"}