{"product_id":"xrcc5-phospho-t714-polyclonal-antibody-bs4240","title":"XRCC5 (phospho-T714) Polyclonal Antibody-BS4240","description":"\u003cp\u003eXRCC5 (phospho-T714) Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS4240-50, BS4240-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P13010\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eXRCC5 (phospho-T714) Polyclonal Antibody-BS4240\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, IHC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB: 1:500~1:1000\u003cbr\u003e\nIHC: 1:50~1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: XRCC5 encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: p-XRCC5 (T714) polyclonal antibody detects endogenous levels of XRCC5 protein only when phosphorylated at Thr714\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: ~ 82 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot (WB) analysis of p-XRCC5 (T714) pAb at 1:500 dilution\u003cbr\u003e\n Lane 1: A549 whole cell lysate (40ug) \u003cbr\u003e\n Lane 2: A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate (40ug) \u003cbr\u003e\n Lane 3: A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate (40ug) \u003cbr\u003e\n Lane 4: The Brain tissue lysate of Mouse (40ug) \u003cbr\u003e\n Lane 5: The Uterus tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of p-Ku-80 (T714) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Ku-80; Ku-86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); XRCC5; G22P2\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Synthetic phosphopeptide derived from human XRCC5 around the phosphorylation site of Threonine 714.\u003c\/p\u003e\n\n\u003cp\u003eModification: Phosphorylation\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957297254579,"sku":"BS4240-50","price":276.71,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957297287347,"sku":"BS4240-100","price":490.83,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_25465ddf-a9f6-4ca7-a18f-67d991902d04.jpg?v=1705144517","url":"https:\/\/afsbio.com\/products\/xrcc5-phospho-t714-polyclonal-antibody-bs4240","provider":"AFSBio Inc.","version":"1.0","type":"link"}