{"product_id":"yy1-polyclonal-antibody-bs75095","title":"YY1 Polyclonal Antibody-BS75095","description":"\u003cp\u003eYY1 Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eProduct: 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS75095-50, BS75095-100\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P25490\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eYY1 Polyclonal Antibody-BS75095\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human, Mouse, Rat\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ChIP,\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB,1:1000 - 1:2000  |  ChIP,1:50 - 1:200\u003c\/p\u003e\n\n\u003cp\u003eBackground: YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is \u0026gt; 95% (by SDS-PAGE).\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Unmodification\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 65KDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of extracts of various cell lines, using YY1 antibody at 1:1000 dilution.\u003cbr\u003e\nSecondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.\u003cbr\u003e\nLysates\/proteins: 25ug per lane.\u003cbr\u003e\nBlocking buffer: 3% non-fat dry milk in TBST.\u003cbr\u003e\nDetection: ECL Basic Kit.\u003cbr\u003e\nExposure time: 180s., Chromatin immunoprecipitation analysis of extracts of 293T cells, using YY1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1; YY1\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant fusion protein of human YY1(NP_003394.1).\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":42957350830259,"sku":"BS75095-50","price":342.59,"currency_code":"CAD","in_stock":true},{"title":"100µl","offer_id":42957350863027,"sku":"BS75095-100","price":589.65,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/thumbnail_d1447bac803ec9be_11-14-09-19-51_5cd8281b-4730-436e-8533-453678116f7e.jpg?v=1705145176","url":"https:\/\/afsbio.com\/products\/yy1-polyclonal-antibody-bs75095","provider":"AFSBio Inc.","version":"1.0","type":"link"}