Product Description

Actin β Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1592-50, ES1592-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat, Chicken, Globefish, Bovine, Hamster, Pig, Ovine, Cat, Pig, Dog

Host: Rabbit

Applications: IF, WB, IHC, ELISA

Alternate Names: ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1-80

Isotype: IgG

Target: Actin β,

Specificity: Actin β Polyclonal Antibody detects endogenous levels of Actin β protein.

Dilution: IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: B-cell lymphoma, Brain, Cajal-Retzius cell, Eye, Fetal brain cortex, Foreskin, Hepatocellular car

Observed Band (KD): 42kD

Fields: Rap1 signaling pathway, Phagosome, Apoptosis, Hippo signaling pathway, Focal adhesion, Adherens junction, Tight junction, Platelet activation, Neutrophil extracellular trap formation, Leukocyte transendothelial migration, Thermogenesis, Regulation of actin cytoskeleton, Thyroid hormone signaling pathway, Oxytocin signaling pathway, Gastric acid secretion, Amyotrophic lateral sclerosis, Bacterial invasion of epithelial cells, Vibrio cholerae infection, Pathogenic Escherichia coli infection, Shigellosis, Salmonella infection, Yersinia infection, Influenza A, Proteoglycans in cancer, Hepatocellular carcinoma, Hypertrophic cardiomyopathy, Arrhythmogenic right ventricular cardiomyopathy, Dilated cardiomyopathy, Viral myocarditis, Fluid shear stress and atherosclerosis

Function: Disease: Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Similarity: Belongs to the

Subcellular Location: Cytoplasm, cytoskeleton Nucleus Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Storage and Stability: -20°C/1 year

Gene Name: ACTB

Protein Name: Actin cytoplasmic 1

Human Gene ID: 60

Human SwissProt Number: P60709

Mouse Gene ID: 11461

Mouse SwissProt Number: P60710

Rat Gene ID: 81822

Rat SwissProt Number: P60711

Research Use Only