As a leading supplier of life science equipment and reagents, AFS Bio works with Canadian researchers, hospitals, and biopharma companies seeking to increase efficiencies, reduce costs, and streamline their workflow in the lab.
Primary antibodies are immunoglobulins that bind directly to antigens. They’re used to detect, quantify, or purify antigens in a sample. Primary antibodies do not come with fluorophores attached – to measure them, a secondary antibody (of a species different from both the primary antibody and sample) must be applied. We offer mouse, rat, pig, bovine, chicken, dog and human monoclonal and polyclonal antibodies to suit your research needs.View Products
Enzyme-linked immunosorbent assay (ELISA) is used to detect and measure the amount of peptides, proteins, antibodies, and hormones in a sample. It’s different from typical antibody assays in that it involves enzyme-linked antibody binding to antigens immobilized on a solid surface – generally a polystyrene multiwell plate – and allows for quantitative analysis of the sample. During incubation, the conjugated enzyme is broken down into a colored product, indicating the amount of analyte present in the sample. ELISA’s provide rapid, easily visualized results, and can be performed on a high number of samples simultaneously.View Products
Secondary antibodies do not bind to the antigen under analysis, but instead to the heavy chain of primary antibodies that perform the direct antigen binding. Attached to secondary antibodies are colorimetric, chemiluminescent, or fluorescent markers that serve as tags for antibody-antigen binding, signaling the amount of antigen present. The secondary antibody must be able to recognize the species from which the primary antibody was extracted, so should be derived from a different species.View Products
Isotype controls are used to verify the binding specificity of a primary antibody. They lack specificity to the target, but belong to the same class and type of primary antibody being applied. Isotype controls are effective at delineating non-specific background signals from specific antibody signals, providing you with a more accurate indication of the degree of antibody binding. As extra background is a common issue with primary antibodies (resulting from binding to Fc receptors on target cells, non-specific interactions with other cellular components, or cellular autofluorescence), isotype staining is a useful means of visualizing the degree of non-specific binding in a sample.View Products