Product Description

AMPKα1/2 (phospho-T183/172) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5003-50, BS5003-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AMPK is a heterotrimeric complex comprising a catalytic α subunit and regμlatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regμlation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regμlate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regμlatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, au-protein kinase PRKAA1, PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human AMPKα1 around the phosphorylation site of Threonine 183.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-AMPKα1/2 (T183/172) polyclonal antibody detects endogenous levels of AMPKα1 protein only when phosphorylated at Thr183 and AMPKα2 protein only when phosphorylated at Thr172.

Molecular Weight: ~ 63 kDa

Pathway: Hypoxia Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, Insμlin &Glucose Signaling, AMPK Signaling Pathway, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13131/P54646

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only