AMPKα1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS80348-50, BS80348-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13131
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IF/ICC
All Applications: IF/ICC,1:50 - 1:20
Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunofluorescence analysis of HeLa cells using AMPKα1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using AMPKα1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using AMPKα1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining.
Alternative Name: PRKAA1; AMPK; AMPKa1; protein kinase AMP-activated catalytic subunit alpha 1
Immunogen: A synthetic peptide of human AMPKα1(NP_006242.5).
Conjugate: Unconjugated
Modification: Unmodified