Product Description

Casein Kinase Iα (phospho Tyr294) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4857-50, ES4857-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CSNK1A1 (Casein Kinase 1 Alpha 1) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Infectious disease. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CSNK1G2. asein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at Ser-45. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed: 11955436, PubMed: 1409656, PubMed: 18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed: 23902688). asein kinase I (CK1) is a monomeric serine-threonine protein kinase with 7 isoforms: alpha, beta, gamma1, gamma2, gamma3, delta and epsilon. CK1 is involved in many cellular processes including DNA repair, cell division, nuclear localization and membrane transport. Isoforms are also integral to development.

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: CSNK1A1, Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1L, Casein kinase I isoform alpha-like, CKI-alpha-like, CK1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human CK-1 alpha around the phosphorylation site of Tyr294. AA range:260-309

Isotype: IgG

Target: Casein Kinase Iα

Specificity: Phospho-Casein Kinase Iα (Y294) Polyclonal Antibody detects endogenous levels of Casein Kinase Iα protein only when phosphorylated at Y294.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Epithelium, Liver, Lung, Placenta, Skin,

Observed Band (KD): 37kD

Fields: Wnt signaling pathway, Hedgehog signaling pathway, Alzheimer disease, Pathways of neurodegeneration - multiple diseases, Human papillomavirus infection, Breast cancer, Hepatocellular carcinoma, Gastric cancer

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Function: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 on 'Ser-45'
Similarity: Belongs to the protein kinase superfamily
Similarity: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily
Similarity: Contains 1 protein kinase domain
Subunit: Monomer. Interacts with the Axin complex

Subcellular Location: Cytoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore Nucleus speckle Cytoplasm, cytoskeleton, cilium basal body Cytoplasm, cytoskeleton, spindle Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis Also recruited to the keratin cytoskeleton (PubMed:23902688)

Storage and Stability: -20°C/1 year

Gene Name: CSNK1A1

Protein Name: Casein kinase I isoform alpha

Rat Gene ID: 113927

Rat SwissProt Number: P97633

Research Use Only