Product Description

IL-10Rα (phospho Tyr496) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5891-50, ES5891-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: IL10RA, IL10R, Interleukin-10 receptor subunit alpha, IL-10 receptor subunit alpha, IL-10R subunit alpha, IL-10RA, CDw210a, Interleukin-10 receptor subunit 1, IL-10R subunit 1, IL-10R1, CD antigen CD210

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human IL-10R alpha around the phosphorylation site of Tyr496. AA range:462-511

Isotype: IgG

Target: IL-10Rα

Specificity: Phospho-IL-10Rα (Y496) Polyclonal Antibody detects endogenous levels of IL-10Rα protein only when phosphorylated at Y496.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages, Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells,

Observed Band (KD): 63kD

Fields: Cytokine-cytokine receptor interaction, Viral protein interaction with cytokine and cytokine receptor, JAK-STAT signaling pathway, Toxoplasmosis, Tuberculosis, Human cytomegalovirus infection

Function: Function: Receptor for IL10, binds IL10 with a high affinity
Similarity: Belongs to the type II cytokine receptor family
Tissue Specificity: Spleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels

Subcellular Location: Cell membrane, Single-pass type I membrane protein Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: IL10RA

Protein Name: Interleukin-10 receptor subunit alpha

Mouse SwissProt Number: Q61727

Research Use Only