Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit - ELK9154

Mouse NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9154-48, ELK9154-96, ELK9154-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: NF kappa B p65delta3; NFKB3

Assay Type: Sandwich

Sensitivity: 12.2 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates

Assay Length: 3.5h

Research Areas: Immunology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q04207

Research Use Only