Phospho-IKKα-S180 Polyclonal Antibody-BS79386

Phospho-IKKα-S180 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79386-50, BS79386-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15111

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-IKKα-S180 antibody at 1:1000 dilution. HeLa cells were treated by both TNF-α and Calyculin A for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.

Bioworld Molecular Weight: 100kDa

Specificity: Phosphorylated

Alternative Name: CHUK; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16; IKK alpha

Immunogen: A synthetic phosphorylated peptide around S180 of human IKKα(NP_001269.3).

Conjugate: Unconjugated

Modification: Phosphorylated