Product Description

PLC γ1 (phospho Tyr1253) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6605-50, ES6605-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: PLCG1, PLC1, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr1253. AA range:1221-1270

Isotype: IgG

Target: PLCG1

Specificity: Phospho-PLC γ1 (Y1253) Polyclonal Antibody detects endogenous levels of PLC γ1 protein only when phosphorylated at Y1253.

Dilution: WB 1:500-2000, IHC-p 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Epithelium, Testis, Vein,

Observed Band (KD): 150kD

Fields: Inositol phosphate metabolism, Metabolic pathways, EGFR tyrosine kinase inhibitor resistance, ErbB signaling pathway, Ras signaling pathway, Rap1 signaling pathway, Calcium signaling pathway, Chemokine signaling pathway, NF-kappa B signaling pathway, HIF-1 signaling pathway, Phosphatidylinositol signaling system, Phospholipase D signaling pathway, Axon guidance, VEGF signaling pathway, Neutrophil extracellular trap formation, Natural killer cell mediated cytotoxicity, Th1 and Th2 cell differentiation, Th17 cell differentiation, T cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Inflammatory mediator regulation of TRP channels, Thyroid hormone signaling pathway, AGE-RAGE signaling pathway in diabetic complications, Growth hormone synthesis, secretion and action, Parkinson disease, Pathways of neurodegeneration - multiple diseases, Vibrio

Function: Catalytic Activity: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Cofactor: Calcium
Domain: The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1
Function: PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase
PTM: The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors
PTM: Ubiquitinated by CBLB in activated T-cells
Similarity: Contains 1 C2 domain
Similarity: Contains 1 EF-hand domain
Similarity: Contains 1 PH domain
Similarity: Contains 1 PI-PLC X-box domain
Similarity: Contains 1 PI-PLC Y-box domain
Similarity: Contains 1 SH3 domain
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Subcellular Location: Cell projection, lamellipodium Cell projection, ruffle Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment

Storage and Stability: -20°C/1 year

Gene Name: PLCG1

Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

Mouse Gene ID: 18803

Mouse SwissProt Number: Q62077

Rat Gene ID: 25738

Rat SwissProt Number: P10686

Research Use Only