Product Description

PLC γ1 (phospho-Y1253) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4865-50, BS4865-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregμlatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regμlates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4, 5-biphosphate. PLC γ1 binds α1β1 Integrin and modμlates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regμlated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ICC

Alternate Names: 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, PLC-148, PLC148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C2, PLC2I, Phospholipase C-gamma-1, PLC-gamma-1, PLCG1, PLC1, PLCγ1,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human PLC γ1 around the phosphorylation site of Tyrosine 1253.

Dilution: WB: 1:500~1:1000
ICC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-PLC γ1 (Y1253) polyclonal antibody detects endogenous levels of PLC γ1 protein when phosphorylated at Tyr1253.

Molecular Weight: ~ 155 kDa

Pathway: Phosphoinosotode Signaling, Phospholipase Signaling, Regμlation of Actin Dynamics, Tumor Angiogenesis, T cell receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P19174

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only