Product Description

PP2A-α (Phospho-Y307) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4867-50, BS4867-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regμlates fundamental cellμlar activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development. The core enzyme consists of catalytic C and regμlatory A (or PR65) subunits, with each subunit represented by α and β isoforms. Additional regμlatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regμlatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regμlatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit. This variable array of holoenzyme components, particμlarly regμlatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regμlated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insμlin and resμlts in a substantial reduction of PP2A activity. Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed. Methylation alters the conformation of PP2A, as well as its localization and association with B regμlatory subunits.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IF

Alternate Names: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, EC:3.1.3.16, PP2A-alpha, Replication protein C, RP-C, PPP2CA

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human PP2A-α around the phosphorylation site of Tyrosine 307.

Dilution: WB: 1:500~1:1000
IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PP2A-α (Phospho-Y307) polyclonal antibody detects endogenous levels of PP2A-α protein only when phosphorylated at Tyr307.

Molecular Weight: ~ 36 kDa

Pathway: Contribution of Soluble Factors to EMT,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P67775

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only