Product Description

14-3-3 sigma (Phospho-S186) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67435-50, BS67435-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The 14-3-3 family of proteins plays a key regμlatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways . 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively . Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecμles . The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed. 14-3-3 binding masks specific sequences of the target protein, and therefore, modμlates target protein localization, phosphorylation state, stability, and molecμlar interactions . 14-3-3 proteins may also induce target protein conformational changes that modify target protein function . Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellμlar signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities . Several studies suggest that 14-3-3 isoforms are differentially regμlated in cancer and neurological syndromes.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep

Host: Rabbit

Applications: WB

Alternate Names: HME1, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 sigma with a site at pS186. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of 14-3-3 sigma with a site at pS186 protein.

Molecular Weight: ~ 35 kDa

Pathway: Regμlation of Actin Dynamics, Regμlation of Apoptosis, Cell Cycle G2 M DNA Damage Signaling Pathway, MAPK Erk in Growth and Differentiation Pathway(, hippo Signaling, Mitochondrial Control of Apoptosis, PI3K AKT signaling, SAPK JNK Signaling Cascades.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P31947

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only