ABflo® 488 Rabbit anti-Human ICAM-1/CD54 Monoclonal Antibody
Sizes: 100T, 2 x 100T, 5 x 100T
Catalogue Numbers: A22312-100, A22312-200, A22312-500
Citations, Manuals and MSDS Available upon request.
Background: Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide . Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier . Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors . CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation . Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway . Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2. SHP-2 seems essential for CD54-induced Src activation.
Category: Monoclonal Antibodies
Application: FC
Cross Reactivity: Human
Protein Weight: 58kDa Immunogen: Recombinant protein (or fragment).
Species: Human
GeneID: 3383
SWISS: P05362
Alternate Names: BB2; CD54; P3.58
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at 2-8℃. Avoid freeze.|Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilution: FC,5 μl per 10^6 cells in 100 μl volume
Research Areas: Cell Biology Developmental Biology, Cell Adhesion, Immunology Inflammation, CDs, Neuroscience, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer's Disease, Neurodegenerative Diseases Markers, Stem Cells, Hematopoietic Progenitors, Cardiovascular
NCBI Alias: ICAM1
Modifications: Unmodified
Conjugate: ABflo® 488. Ex:491nm. Em:516nm.
Clone Number Info: ARC54998
Research Use Only