ACAT1 (K266) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS3796-50, BS3796-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P24752
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: ACAT-1 (acetyl-Coenzyme A acetyltransferase 1) is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. Rare defects in the gene encoding ACAT-1 lead to β-ketothiolase deficiency, which is characterized by ketoacidotic attacks. ACAT-2 (acetyl-Coenzyme A acetyltransferase 2) is considered a cytosolic protein and is crucial for cholesterol synthesis. Specifically, both Acetoacetyl-CoA specific thiolases, ACAT-1 and ACAT-2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction, the cleavage of acetoacetyl-CoA into two acetyl-CoA molecules.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ACAT1 (K266) polyclonal antibody detects endogenous levels of ACAT1 protein.
Bioworld Molecular Weight: ~ 45 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ACAT-1 (K266) pAb at 1:1000 dilution
Lane 1: HEK293T whole cell lysate (20ug)
Lane 2: HepG2 whole cell lysate (20ug)
Lane 3: The Lung tissue lysate of Rat (20ug)
Lane 4: The Lung tissue lysate of Mouse (10ug), Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human kidney carcinoma tissue at 1:50, showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Alternative Name: Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; ACAT1; ACAT; MAT; ACAT-1
Immunogen: Synthetic peptide, corresponding to amino acids 235-286 of Human ACAT1.
Conjugate: Unconjugated
Modification: Unmodified