Product Description

Acid Phosphatase (ACP) Activity Assay Kit (PNPP Method)

Sizes: 96T, 500Assays

Catalogue Numbers: E-BC-K010-M-96, E-BC-K010-M-500

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Disodium p-nitrobenzene phosphate (p-NPP), a widely used phosphatase chromogenic substrate, can form p- nitrophenol under the action of acid phosphatase. Under alkaline conditions, p-nitrophenol is yellow and has a maximum absorption peak at 405 nm. The darker of the yellow product is, the higher of the ACP activity is. Therefore, the activity of ACP can be calculated by measuring the OD value at 405 nm.

Abbreviation: ACP

Target Synonym: ACP

Research Areas: Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.2-50 U/L

Tested Sample Types: Serum;Plasma;Tissue

Assay Length: 30 min

Sensitivity: 0.2 U/L

Inter cv: 4.5%

Intra cv: 2.8%

Research Use Only