Product Description

ACK (phospho Tyr284) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4434-50, ES4434-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: TNK2, ACK1, Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ACK1 around the phosphorylation site of Tyr284. AA range:250-299

Isotype: IgG

Target: ACK

Specificity: Phospho-ACK (Y284) Polyclonal Antibody detects endogenous levels of ACK protein only when phosphorylated at Y284.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i,e, normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages, It also shows a significant increase in expression in prostate cancers during the progressive stages,

Observed Band (KD): 120kD

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Cofactor: Magnesium
Enzyme Regulation: The SH3 domain appears to play an autoinhibitory role
Function: Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development
Sequence Caution: Unlikely isoform. Aberrant splice sites
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family
Similarity: Contains 1 CRIB domain
Similarity: Contains 1 protein kinase domain
Similarity: Contains 1 SH3 domain
Subunit: Interacts with CDC42. Interacts with activated CSPG4

Subcellular Location: Cell membrane Nucleus Endosome Cell junction, adherens junction Cytoplasmic vesicle membrane, Peripheral membrane protein, Cytoplasmic side Cytoplasmic vesicle, clathrin-coated vesicle Membrane, clathrin-coated pit Cytoplasm, perinuclear region Cytoplasm, cytosol The Tyr-284 phosphorylated form is found both in the membrane and nucleus (By similarity) Co-localizes with EGFR on endosomes (PubMed:20333297) Nuclear translocation is CDC42-dependent (By similarity) Detected in long filamentous cytosolic structures where it co-localizes with CTPS1 (By similarity)

Storage and Stability: -20°C/1 year

Gene Name: TNK2

Protein Name: Activated CDC42 kinase 1

Mouse Gene ID: 51789

Mouse SwissProt Number: O54967

Research Use Only