ACSL1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS76706-50, BS76706-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P33121
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC, IP,
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200
Background: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Unmodification
Bioworld Molecular Weight: 78KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ACSL1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of C6 cells using ACSL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH-3T3 cells using ACSL1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 300ug extracts of Raji cells using 3ug ACSL1 antibody. Western blot was performed from the immunoprecipitate using ACSL1 antibody at a dilution of 1:1000.
Alternative Name: ACS1; FACL1; FACL2; LACS; LACS1; LACS2; ACSL1
Immunogen: Recombinant fusion protein of human ACSL1(NP_001986.2).
Conjugate: Unconjugated
Modification: Unmodified