Product Description

Actinin-α1/2/3/4 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1593-50, ES1593-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: ACTN1, Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN2, Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN3, Alpha-actinin-3, A

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ACTN1/2/3/4. AA range:21-70

Isotype: IgG

Target: Actinin-α1/2/3/4

Specificity: Actinin-α1/2/3/4 Polyclonal Antibody detects endogenous levels of Actinin-α1/2/3/4 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Colon, Epithelium, Hepatocyte, Neuroblastoma, Placenta, Platelet, Skin, Tumor of tongue,

Observed Band (KD): 75kD

Fields: Focal adhesion, Adherens junction, Tight junction, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Shigellosis, Amoebiasis, Viral carcinogenesis, Systemic lupus erythematosus

Function: Function: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein
Similarity: Belongs to the alpha-actinin family
Similarity: Contains 1 actin-binding domain
Similarity: Contains 2 CH (calponin-homology) domains
Similarity: Contains 2 EF-hand domains
Similarity: Contains 4 spectrin repeats
Subcellular Location: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle
Subunit: Homodimer, antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP

Subcellular Location: Cytoplasm, cytoskeleton Cytoplasm, myofibril, sarcomere, Z line Cell membrane Cell junction Cell projection, ruffle Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle Colocalizes with PSD in membrane ruffles and central reticular structures

Storage and Stability: -20°C/1 year

Gene Name: ACTN1/2/3/4

Protein Name: Alpha-actinin-1/2/3/4

Mouse Gene ID: 109711/11472/11474/60595

Rat Gene ID: 81634/63836

Rat SwissProt Number: Q9Z1P2/Q9QXQ0

Research Use Only