Product Description

Actinin-α3 (E25) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2205-50, BS2205-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellμlar structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerμlosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and,μltimately, renal failure.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF

Alternate Names: Alpha-actinin-3, Alpha-actinin skeletal muscle isoform 3, ACTN3, α-Actinin3, Actinin α3, Actininα3, αActinin-3, Actinin-α3, α-actinin-3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Actinin-α3.

Dilution: WB: 1:500~1:1000
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Actinin-α3 (E25) polyclonal antibody detects endogenous levels of actinin-α3 protein, this antibody does not cross-react with other α-actinin isoforms.

Molecular Weight: ~ 103 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q08043

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only