ADAMTS1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS72575-50, BS72575-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UHI8
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200
Background: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 105kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of various cell lines, using ADAMTS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of 293F cells using ADAMTS1 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining., Immunofluorescence analysis of HeLa cells using ADAMTS1 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using ADAMTS1 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining.
Alternative Name: ADAMTS1; C3-C5; METH1
Immunogen: Recombinant fusion protein of human ADAMTS1(NP_008919.3).
Conjugate: Unconjugated
Modification: Unmodified