ADSL Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS8096-50, BS8096-100
Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Swiss-Prot: P30566
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ELISA IHC IF
All Applications: WB: 1:500-1:2000
ELISA: 1:5000-1:10000
IHC: 1:100-1:500
IF: 1:100-1:500
Background: ADSL (adenylosuccinate lyase), also known as AMPS, ASL or ASASE, is a 484 amino acid protein that is involved in both purine biosynthesis and in the formation of adenosine monophosphate (AMP) from inosine monophosphate. Expressed ubiquitously, ADSL catalyzes two key reactions in AMP biosynthesis, namely the removal of a fumarate from succinylaminoimidazole carboxamide (SAICA) ribotide to give aminoimidazole carboxamide ribotide (AICA) and the subsequent removal of fumarate from adenylosuccinate to yield AMP. Defects in the gene encoding ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency), an autosomal recessive disorder characterized by epilepsy, growth retardation and muscular wasting. Multiple isoforms of ADSL exist due to alternative splicing events.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ADSL polyclonal antibody detects endogenous levels of ADSL protein.
Bioworld Molecular Weight: ~ 50 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Primary: Anti-Adenylosuccinate Lyase at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated.at 1:200 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adenylosuccinate Lyase) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.
Alternative Name: Adenylosuccinate lyase; ASL; Adenylosuccinase; Asase; ADSL; AMPS
Immunogen: KLH conjugated synthetic peptide derived from human Adenylosuccinate Lyase: 185-280/484
Conjugate: Unconjugated
Modification: Unmodified