Product Description

AIFM1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66693-50, MB66693-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimμli. Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimμli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis. Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain. While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis. Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: AIF, PDCD8, Apoptosis-inducing factor 1 mitochondrial, Programmed cell death protein 8

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human AIF protein fragments expressed in E.coli.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of AIFM1 protein.

Molecular Weight: ~ 67 kDa

Pathway: Regμlation of Apoptosis, Mitochondrial Control of Apoptosis.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O95831

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only