Product Description

AKT Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66653-50, MB66653-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insμlin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regμlator of the PI3K/Akt signaling pathway. LY294002 is a specific PI3 kinase inhibitor. Another essential Akt function is the regμlation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β. Akt may also play a role in insμlin stimμlation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regμlation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 and by negatively regμlating the cyclin-dependent kinase inhibitors p27 Kip1 and p21 Waf1/Cip1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB, IP

Alternate Names: AKT1, PKB, RAC, RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT2, RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT3, PKBG, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant human AKT1 protein fragments expressed in E.coli.AKT1 interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10, the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding By similarity. Interacts with AGAP2 (isoform 2/PIKE-A), the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B, the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13, the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.AKT2 interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. AKT3 interacts (via PH domain) with TCL1A, this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of AKT protein.

Molecular Weight: ~ 60 kDa

Pathway: AMPK Signaling Pathway, Adherens Junction signaling, Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimer\'s Disease, Tumor Angiogenesis, Regμlation of Apoptosis, ESC Pluripotency and Differentiation Signaling Pathway, ErbB HER Signaling, Cell Cycle Control G1 S Checkpoin, Jak Stat: IL-6 Receptor Signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Regμlation of Microtubμle Dynamics, Mitochondrial Control of Apoptosis, NF-kB Signaling, PI3K AKT signaling, Protein Kinase C Signaling, T cell receptor signaling, TGFβ Smad signaling, Translational Contral elF4 and p70 S6 Kinase, Warburg Effect, MTOR signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P31749, P31751, Q9Y243

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only