Product Description

AKT2 (phospho-S474) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4720-50, BS4720-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regμlatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regμlator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2, PKBβ,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-AKT2 (S474) polyclonal antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474.This antibody does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.

Molecular Weight: ~ 60 kDa

Pathway: Phosphoinosotode Signaling, PI3K AKT signaling, Immune Cell Signaling, Warburg Effect, Translational Contral elF4 and p70 S6 Kinase, T cell receptor signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P31751

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only