Product Description

Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP Method)

Sizes: 96T, 500Assays

Catalogue Numbers: E-BC-K009-M-96, E-BC-K009-M-500

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Under alkaline conditions, alkaline phosphatase catalyzes the hydrolysis of p-nitrobenzene phosphate disodium to produce p-nitrophenol and phosphoric acid. Under strong alkaline conditions, p-nitrophenol is bright yellow and has a maximum absorption peak at 405 nm. Therefore, the activity of ALP can be calculated by measuring the OD value at 405 nm.

Abbreviation: ALP

Target Synonym: ALP

Research Areas: Liver and Renal Function

Result Type: Enzyme activity

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Detection Range: 0.27-50.8 U/L

Tested Sample Types: Serum;Plasma;Animal tissue;Cell

Assay Length: 30 min

Sensitivity: 0.27 U/L

Inter cv: 4.6%

Intra cv: 1.2%

Research Use Only