ALOX5 (E267) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS1506-50, BS1506-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P09917
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC
All Applications: IHC: 1:50~1:200
Background: 5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells. 5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis. The cellular localization of 5-LO varies between cell types. In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope. In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry (IHC) analyzes of ALOX5 (E267) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50,showing cytoplasm,nucleus and nucleus membrane staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Bioworld Molecular Weight: ~ 78 kDa
Specificity: ALOX5 (E267) polyclonal antibody detects endogenous levels of ALOX5 protein.
Alternative Name: Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase; ALOX5; LOG5
Immunogen: Synthetic peptide, corresponding to amino acids 231-280 of Human ALOX5.
Conjugate: Unconjugated
Modification: Unmodified