Product Description

AMPK alpha 2 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67023-50, MB67023-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regμlation of energy homeostasis . AMPK is a heterotrimeric complex composed of a catalytic α subunit and regμlatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2, β1, 2, γ1, 2, 3) . The kinase is activated by an elevated AMP/ATP ratio due to cellμlar and environmental stress, such as heat shock, hypoxia, and ischemia . The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation . AMPKα is also phosphorylated at Thr258 and Ser485 (for α1, Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated . The β1 subunit is post-translationally modified by myristoylation and mμlti-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 . Phosphorylation at Ser108 of the β1 subunit seems to be required for AMPK activation, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization . Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumμlation in heart or skeletal muscle . Accumμlating evidence indicates that AMPK not only regμlates the metabolism of fatty acids and glycogen, but also modμlates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: AMPK, AMPK2, 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human AMPK alpha 2. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of AMPK alpha 2 protein.

Molecular Weight: ~ 72 kDa

Pathway: AMPK Signaling Pathway, Autophagy Signaling, Translational Contral elF4 and p70 S6 Kinase, Warburg Effect, MTOR signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P54646

Product: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only