Annexin A11 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS8886-50, BS8886-100
Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P50995
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IF/ICC, IP,
All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:100
Background: This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Polyclonal Antibodies
Bioworld Molecular Weight: 56KDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of extracts of Rat heart cells, using Annexin A11 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of PC-12 cells using Annexin A11 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using Annexin A11 Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Annexin A11 antibody. Western blot was performed from the immunoprecipitate using Annexin A11 antibody at a dilution of 1:1000.
Alternative Name: ANXA11; ANX11; CAP50
Immunogen: Recombinant fusion protein of human Annexin A11 (NP_001148.1).
Conjugate: Unconjugated
Modification: Unmodified