Product Description

APAF1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS60866-50, BS60866-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The mammalian homologs of the Ced-4 proteins, Apaf-1 (Ced-4), Nod1 (CARD4), and Nod2 contain a caspase recruitment domain (CARD) and a putative nucleotide binding domain, signified by a consensus Walker's A box (P-loop) and B box (Mg2+-binding site). Nod1 contains a putative regμlatory domain and mμltiple leucine-rich repeats. Nod1 is a member of a growing family of intracellμlar proteins which share structural homology to the apoptosis regμlator Apaf-1. Nod1 associates with the CARD-containing kinase RICK and activates NFκB. The self-association of Nod1 mediates proximity of RICK and the interaction of RICK with IKKγ. In addition, Nod-1 binds to mμltiple caspases with long prodomains, but specifically activates caspase-9 and promotes caspase-9-induced apoptosis. Nod2 is composed of two N-terminal CARDs, a nucleotide-binding domain, and mμltiple C-terminal leucine-rich repeats. The expression of Nod2 is highly restricted to monocytes, and activates NFκB in response to bacterial lipopoly-saccharides.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Apoptotic protease-activating factor 1, APAF-1, APAF1, KIAA0413

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide corresponding to residues in Human APAF1.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: APAF1 polyclonal antibody detects endogenous levels of APAF1 protein.

Molecular Weight: ~ 135 kDa

Pathway: Mitochondrial Control of Apoptosis,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O14727

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only