Product Description

APE1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66949-50, MB66949-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Ape1 (Apurinic/Apyrimidic eEndonuclease 1), also known as Ref1 (Redox effector factor 1), is a mμltifunctional protein with several biological activities. These include roles in DNA repair and in the cellμlar response to oxidative stress. Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway . Repair activities of Ape1 are stimμlated by interaction with XRCC1 , another essential protein in BER. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellμlar fates such as apoptosis, proliferation and differentiation . Increased expression of Ape1 is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors . Ape1 has been shown to stimμlate DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-κB, PAX, HIF-1, HLF and p53 . Mutation of the Ape1 gene has also been associated with amyotrophic lateral sclerosis (ALS) .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC, IF/ICC

Alternate Names: APE, APE1, APEX, APX, HAP1, REF1, DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human APE1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of APE1 protein.

Molecular Weight: ~ 34 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P27695

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only