Product Description

APOBEC3A Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5099-50, ES5099-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided b

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: APOBEC3A, Probable DNA dC->dU-editing enzyme APOBEC-3A, Phorbolin-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human APOBEC3A. AA range:27-76

Isotype: IgG

Target: APOBEC3A

Specificity: APOBEC3A Polyclonal Antibody detects endogenous levels of APOBEC3A protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells, Highly expressed in keratinocytes and in periphery blood monocytes, Also detected in non-lymphoid tissues including lung and adipose tissues, Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia,

Observed Band (KD): 26kD

Fields: Viral life cycle - HIV-1, Human immunodeficiency virus 1 infection

Function: Cofactor: Zinc
Function: Lacks cytidine deaminase activity, at least on RNA molecules (monomeric nucleoside substrates or synthetic apoB RNA template). Unable to reduce HIV-1 infectivity in vitro
Miscellaneous: It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22
Similarity: Belongs to the cytidine and deoxycytidylate deaminase family
Tissue Specificity: Expressed in peripherical leukocytes and keratinocytes

Subcellular Location: Nucleus Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: APOBEC3A

Protein Name: Probable DNA dC->dU-editing enzyme APOBEC-3A

Research Use Only