Product Description

APS Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4517-50, ES4517-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway. [provided by RefSeq, Jun 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: SH2B2, APS, SH2B adapter protein 2, Adapter protein with pleckstrin homology and Src homology 2 domains, SH2 and PH domain-containing adapter protein APS

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from the Internal region of human APS.

Isotype: IgG

Target: APS

Specificity: APS Polyclonal Antibody detects endogenous levels of APS protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in spleen, prostate, testis, uterus, small intestine and skeletal muscle, Among hematopoietic cell lines, expressed exclusively in B-cells, Not expressed in most tumor cell lines,

Observed Band (KD): 67kD

Fields: Neurotrophin signaling pathway, Insulin signaling pathway

Function: Function: Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. May be involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. May induce cytoskeletal reorganization via interaction with VAV3
PTM: Tyrosine phosphorylated by JAK2, KIT and other kinases activated by B-cell receptor in response to stimulation with cytokines, IL3, IL5, PDGF, IGF1, IGF2, CSF2/GM-CSF and cross-linking of the B-cell receptor complex
Similarity: Belongs to the SH2B adapter family
Similarity: Contains 1 PH domain
Similarity: Contains 1 SH2 domain
Subcellular Location: Cytoplasmic before PDGF stimulation. After PDG

Subcellular Location: Cytoplasm Cell membrane Cytoplasmic before PDGF stimulation After PDGF stimulation, localized at the cell membrane and peripheral region

Storage and Stability: -20°C/1 year

Gene Name: SH2B2

Protein Name: SH2B adapter protein 2

Mouse Gene ID: 23921

Mouse SwissProt Number: Q9JID9

Rat Gene ID: 114203

Rat SwissProt Number: Q9Z200

Research Use Only