Product Description

AR-β2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1713-50, ES1713-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, FCM, ELISA

Alternate Names: ADRB2, ADRB2R, B2AR, Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor beta2. AA range:321-370

Isotype: IgG

Target: Adrenergic Receptor β2

Specificity: AR-β2 Polyclonal Antibody detects endogenous levels of AR-β2 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. Flow cytometry: 1/200 - 1/400. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Blood, Brain, Fetal brain, Heart, Leukocyte, Prostate, Thyroid,

Observed Band (KD): 47kD

Fields: Calcium signaling pathway, cGMP-PKG signaling pathway, cAMP signaling pathway, Neuroactive ligand-receptor interaction, Adrenergic signaling in cardiomyocytes, Regulation of lipolysis in adipocytes, Renin secretion, Salivary secretion, Chemical carcinogenesis - receptor activation

Function: Disease: Polymorphic forms of ADRB2 could impart some form of nocturnal asthma
Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine
PTM: Palmitoylated, may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation
PTM: Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR
PTM: Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the recep

Subcellular Location: Cell membrane, Multi-pass membrane protein Early endosome Golgi apparatus Colocalizes with VHL at the cell membrane (PubMed:19584355) Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325) Activated receptors are also detected within the Golgi apparatus (PubMed:27481942)

Storage and Stability: -20°C/1 year

Gene Name: ADRB2

Protein Name: Beta-2 adrenergic receptor

Mouse SwissProt Number: P18762

Research Use Only