Product Description

AR Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4268-50, ES4268-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoform

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IF, WB, IHC, ELISA

Alternate Names: AR, DHTR, NR3C4, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from the Internal region of human AR.

Isotype: IgG

Target: Androgen Receptor

Specificity: AR Polyclonal Antibody detects endogenous levels of AR protein.

Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: [Isoform 2]: Mainly expressed in heart and skeletal muscle,, [Isoform 3]: Expressed in basal and stromal cells of the prostate (at protein level),

Observed Band (KD): 99kD

Fields: Oocyte meiosis, Pathways in cancer, Chemical carcinogenesis - receptor activation, Prostate cancer

Function: Disease: Defects in AR are the cause of androgen insensitivity syndrome (AIS) [MIM: 300068], previously known as testicular feminization syndrome (TFM). AIS is an X-linked recessive form of pseudohermaphroditism due end-organ resistance to androgen. Affected males have female external genitalia, female breast development, blind vagina, absent uterus and female adnexa, and abdominal or inguinal testes, despite a normal 46,XY karyotype
Disease: Defects in AR are the cause of androgen insensitivity syndrome partial (PAIS) [MIM: 312300], also known as Reifenstein syndrome. PAIS is characterized by hypospadias, hypogonadism, gynecomastia, genital ambiguity, normal XY karyotype, and a pedigree pattern consistent with X-linked recessive inheritance. Some patients present azoospermia or severe oligospermia without other clinical manifestations
Disease: Defects in AR are the cause of spinal and bulb

Subcellular Location: Nucleus Cytoplasm Detected at the promoter of target genes (PubMed:25091737) Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding Can also translocate to the nucleus in unligated form in the presence of RACK1

Storage and Stability: -20°C/1 year

Gene Name: AR

Protein Name: Androgen receptor

Mouse Gene ID: 11835

Mouse SwissProt Number: P19091

Rat Gene ID: 24208

Rat SwissProt Number: P15207

Research Use Only