Product Description

ASC Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1715-50, ES1715-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: PYCARD; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; hASC; Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ASC. AA range:10-59

Isotype: IgG

Target: ASC,

Specificity: ASC Polyclonal Antibody detects endogenous levels of ASC protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Expressed in lung epithelial cells (at protein level) (PubMed:23229815). Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.

Observed Band (KD): 21kD

Fields: Necroptosis, NOD-like receptor signaling pathway, Cytosolic DNA-sensing pathway, C-type lectin receptor signaling pathway, Pathogenic Escherichia coli infection, Shigellosis, Salmonella infection, Pertussis, Legionellosis, Yersinia infection, Influenza A, Lipid and atherosclerosis

Function: Domain: Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase 9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. miscellaneous:In breast tumorigenesis, methylation-mediated silencing may affect genes and proteins that act as positive mediators of cell death. PTM: Phosphorylated. Similarity: Contains 1 CARD domain. Similarity: Contains 1 DAPIN domain. Subcellular Location: Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. Subunit: Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PY

Subcellular Location: Cytoplasm Inflammasome Endoplasmic reticulum Mitochondrion Nucleus Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures (PubMed:11103777, PubMed:12191486, PubMed:15030775). Upon NLRP3 inflammasome activation redistributes to the perinuclear space localizing to endoplasmic reticulum and mitochondria (PubMed:12191486, PubMed:15030775). Localized primarily to the nucleus in resting monocytes/macrophages and rapidly redistributed to the cytoplasm upon pathogen infection (PubMed:19234215). Localized to large cytoplasmic aggregate appearing as a speck containing AIM2, PYCARD, CASP8 and bacterial DNA after infection with Francisella tularensis (By similarity)., Golgi apparatus

Storage and Stability: -20°C/1 year

Gene Name: PYCARD

Protein Name: Apoptosis-associated speck-like protein containing a CARD

Human Gene ID: 29108

Human SwissProt Number: Q9ULZ3

Mouse Gene ID: 66824

Mouse SwissProt Number: Q9EPB4

Research Use Only