Product Description

ASK1 (phospho-S966) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5055-50, BS5055-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mitogen-activated protein (MAP) kinase cascades are activated by various extracellμlar stimμli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways. ASK 1 Serine 83 is phosphorylated by Akt..

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC

Alternate Names: Apoptosis signal regμlating kinase 1, Apoptosis signal-regμlating kinase 1, ASK 1, ASK-1, ASK1, M3K5, MAP/ERK kinase kinase 5, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Mitogen activated protein kinase kinase kinase 5, Mitogen-activated protein kinase kinase kinase 5,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human ASK1 around the phosphorylation site of Serine 966.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-ASK1 (S966) polyclonal antibody detects endogenous levels of ASK1 protein only when phosphorylated at Ser966.

Molecular Weight: ~ 155 kDa

Pathway: SAPK JNK Signaling Cascades, Signaling Pathways Activating p38 MAP Kinase

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q99683

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only