Product Description

ATAD3A/ATAD3B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS78318-50, BS78318-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q5T9A4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 68KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various lysates, using ATAD3A/ATAD3B antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of U20S cells using ATAD3A/ATAD3B antibody at dilution of 1:100.Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using ATAD3A/ATAD3B antibody at dilution of 1:100.Blue: DAPI for nuclear staining., Immunofluorescence analysis of H9C2 cells using ATAD3A/ATAD3B antibody at dilution of 1:100.Blue: DAPI for nuclear staining.

Alternative Name: ATAD3B; AAA-TOB3; TOB3; ATPase family

Immunogen: Recombinant fusion protein of human ATAD3B(NP_114127.3).

Conjugate: Unconjugated

Modification: Unmodified