Product Description

ATF-1 (phospho Ser63) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6326-50, ES6326-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: activating transcription factor 1(ATF1) Homo sapiens This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chro

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Alternate Names: ATF1, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human ATF1 around the phosphorylation site of Ser63. AA range:31-80

Isotype: IgG

Target: ATF-1

Specificity: Phospho-ATF-1 (S63) Polyclonal Antibody detects endogenous levels of ATF-1 protein only when phosphorylated at S63.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Bone marrow,

Observed Band (KD): 29kD

Fields: Aldosterone synthesis and secretion, Transcriptional misregulation in cancer

Function: Disease: A chromosomal aberration involving ATF1 is associated with angiomatoid fibrous histiocytoma (AFH) [MIM: 612160]. Translocation t(12, 16)(q13, p11.2) with FUS generates a chimeric ATF1/FUS protein
Disease: A chromosomal aberration involving ATF1 is associated with angiomatoid fibrous histiocytoma (AFH) [MIM: 612160]. Translocation t(12, 22)(q13, q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein
Function: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes
Similarity: Belongs to the bZIP family. ATF subfamily
Similarity: Contains 1 bZIP domain
Similarity: Contains 1 KID (kinase-inducible) domain
Subunit: Binds DNA as a dimer

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: ATF1

Protein Name: Cyclic AMP-dependent transcription factor ATF-1

Mouse Gene ID: 11908

Mouse SwissProt Number: P81269

Research Use Only