Product Description

ATP4A Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES10038-50, ES10038-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from human protein . at AA range: 800-880

Isotype: IgG

Target: ATP4A,

Specificity: ATP4A Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Expressed in gastric parietal cells (at protein level).

Observed Band (KD): 113kD

Fields: Oxidative phosphorylation, Metabolic pathways, Collecting duct acid secretion, Gastric acid secretion

Function: Catalytic Activity: ATP + H(2)O + H(+)(In) + K(+)(Out) = ADP + phosphate + H(+)(Out) + K(+)(In). Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. Similarity: Belongs to the cation transport ATPase (P-type) family. Similarity: Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily. Subunit: Composed of two subunits: alpha (catalytic) and beta. Tissue Specificity: Found in gastric mucosa.

Subcellular Location: Apical cell membrane, Multi-pass membrane protein Localized in the apical canalicular membrane of parietal cells (PubMed:24188822). Localized in the apical canalicular membrane of parietal cells (PubMed:24188822).

Storage and Stability: -20°C/1 year

Gene Name: ATP4A

Protein Name: Potassium-transporting ATPase alpha chain 1, EC 3.6.3.10, Gastric H(+ /K(+ ATPase subunit alpha, Proton pump

Human Gene ID: 495

Human SwissProt Number: P20648

Mouse SwissProt Number: Q64436

Rat SwissProt Number: P09626

Research Use Only