ATP6V0A1 Polyclonal Antibody-BS76205

ATP6V0A1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76205-50, BS76205-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q93050

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of HeLa using ATP6V0A1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 using ATP6V0A1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of C6 using ATP6V0A1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: ATP6V0A1; ATP6N1; ATP6N1A; St V1; VPP1; Vph1; a1

Immunogen: Recombinant fusion protein of human ATP6V0A1(NP_001123493.1).

Conjugate: Unconjugated

Modification: Unmodified