Product Description

Axin-2 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67312-50, BS67312-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Axin1 (Axis inhibition protein 1) and Axin2 are mμltidomain scaffold proteins that negatively regμlate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin . Upon stimμlation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling . In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) . Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice . Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop .

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: Axin-2, Axin-like protein, Axil, Axis inhibition protein 2, Conductin

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length protein of human Axin-2

Dilution: WB (1/500 - 1/2000), IF/ICC (1/50 - 1/200)

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Axin-2 protein.

Molecular Weight: ~ 91 kDa

Pathway: ESC Pluripotency and Differentiation Signaling Pathway, Wntβ catenin signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9Y2T1

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only