Product Description

B-RAF Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66889-50, MB66889-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: A-Raf, B-Raf, and c-Raf are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway . Activation of c-Raf is the best understood and involves phosphorylation at mμltiple activating sites, including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 . p21-activated kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A-Raf and B-Raf , although this site is constitutively phosphorylated in B-Raf . Inhibitory 14-3-3 binding sites on c-Raf can be phosphorylated by Akt and AMPK, respectively . While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regμlation has been observed. Of particμlar interest, B-Raf contains three consensus Akt phosphorylation sites and lacks a site equivalent to Tyr341 of c-Raf . Research studies have shown that the B-Raf mutation V600E resμlts in elevated kinase activity and is commonly found in malignant melanoma . Six residues of c-Raf become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: BRAF1, RAFB1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human B-RAF. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of B-RAF protein.

Molecular Weight: ~ 86 kDa

Pathway: ESC Pluripotency and Differentiation Signaling Pathway, ErbB HER Signaling, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, Jak Stat: IL-6 Receptor Signaling, Inhibition of Apoptosis, T cell receptor signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P15056

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only