Product Description

B23 (phospho Thr199) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6413-50, ES6413-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: NPM1, NPM, Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220

Isotype: IgG

Target: Nucleophosmin

Specificity: Phospho-B23 (T199) Polyclonal Antibody detects endogenous levels of B23 protein only when phosphorylated at T199.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Amnion, B-cell lymphoma, Bone marrow, Brain, Cervix carcinoma, Colon carcinoma, Epithelium, Kidney

Observed Band (KD): 32kD

Function: Disease: A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3, 5)(q25.1, q34) with MLF1
Disease: A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5, 17)(q32, q11) with RARA
Disease: A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2, 5)(p23, q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated
Disease: Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location
Function: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressor

Subcellular Location: Nucleus, nucleolus Nucleus, nucleoplasm Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML Can shuttle between cytoplasm and nucleus Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus Colocalized with nucleolin and APEX1 in nucleoli Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis

Storage and Stability: -20°C/1 year

Gene Name: NPM1

Protein Name: Nucleophosmin

Mouse Gene ID: 18148

Mouse SwissProt Number: Q61937

Rat Gene ID: 25498

Rat SwissProt Number: P13084

Research Use Only