Product Description

BAD (Phospho-S155) Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67133-50, MB67133-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellμlar signaling pathways that resμlt in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB

Alternate Names: BBC6, BCL2L8, Bcl2 antagonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-XL/Bcl-2-associated death promoter

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic peptide of human BAD (pS155). The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of BAD (pS155) protein.

Molecular Weight: ~ 23 kDa

Pathway: Regμlation of Apoptosis，ErbB HER Signaling，MAPK Erk in Growth and Differentiation Pathway(，Inhibition of Apoptosis，Mitochondrial Control of Apoptosis，PI3K AKT signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q92934

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only